Tumor marker detection by aptamer-functionalized graphene oxide.


Ming-Yu Lin, Yen-Pei Lu, Alexandru Mihai Grumezescu, Fu Han Ho, Ying-Hsien Kao, Yuh-Shyong Yang, Chih-Hui Yang: Tumor marker detection by aptamer-functionalized graphene oxide. Current Organic Chemistry, 17(2):132-136, 2013.


Interaction of the single-stranded DNA (ssDNA) aptamers and proteins was investigated on graphene oxide (GO)-based biosensors. GO was applied for biological applications because of its unique properties, which include water dispensability, exclusive selection of ssDNA, and fluorescence-quenching effects. In this study, aptamer-functionalized GO sheets of nanometer-scale thickness and micrometer-scale dimension were developed. Based on a simple mixed-and-measured strategy, platelet-derived growth factors (PDGF), the tumor marker of ovarian cancer, triggered aptamers conformation switched and deliberated aptamers from GO surface according to thermal dynamic analysis. By functionalizing with various aptamers on GO, the strategy could provide designed functions on GO-based biosensors, bioreactors, and other devices.