Valentina Grumezescu, Alina Maria Holban, Florin Iordache, Gabriel Socol, George Dan Mogosanu, Alexandru Mihai Grumezescu*, Anton Ficai, Bogdan Stefan Vasile, Roxana Trușcă, Carmen Mariana Chifiriuc, Horia Maniu: MAPLE fabricated magnetite@eugenol and (3-hidroxybutyric acid-co-3-hidroxyvaleric acid) – polyvinyl alcohol microspheres coated surfaces with anti-microbial properties. Applied Surface Science, accepted, 2014.
This study reports the biological applications of a newly fabricated water dispersible nanostructure, basedon magnetite (Fe3O4) and eugenol (E), prepared in a well-shaped spherical form by precipitation method.The presence of Fe3O4@E nanoparticles has been confirmed by transmission electron microscopy (TEM).Nanoparticles have been embedded into poly(3-hidroxybutyric acid-co-3-hidroxyvaleric acid)–polyvinylalcohol (P(3HB-3HV)–PVA) microspheres by oil-in-water emulsion technique. Functionalized P(3HB-3HV)–PVA–Fe3O4@E microspheres coatings have been fabricated by matrix assisted pulsed laserevaporation (MAPLE). The coatings have been characterized by infrared microscopy (IRM) and scan-ning electron microscopy (SEM). In vitro biofilm formation by Staphylococcus aureus and Pseudomonasaeruginosa was assessed by the viable cell counts technique. Nanomaterial biocompatibility has beeninvestigated by analyzing the phenotypic changes of cultured eukaryotic cells. Besides their excellentanti-adherence and anti-biofilm properties, the MAPLE coatings have the advantages of using bioactivenatural compounds, which are less toxic and easily biodegradable than current antibiotics. This approachcould be used as a successful alternative or adjuvant method to control and prevent microbial biofilmsassociated infections.