Crina Saviuc, Alexandru Mihai Grumezescu, Mariana Carmen Chifiriuc, Coralia Bleotu, George Stanciu, Radu Hristu, Dan Eduard Mihaiescu, Veronica Lazar: In vitro methods for the study of microbial biofilms. Biointerface Research in Applied Chemistry, 1(1):31, 2011.
The emergence of microbial biofilm related infections (bacterial and fungal) has a significant impact for the human pathology in the entire world. The understanding of microbial infections related to the biofilm development on tissues or indwelling devices was possible by using different qualitative and quantitative in vitro assays, in continuous and discontinuous systems, as well as in vivo models. A necessary step for obtaining more standardized, reliable and comparable results among different laboratories is the simplification of the available techniques used for investigating the biofilm formation and properties, including the biofilms susceptibility to antibiotics. The aim of the present study was to exemplify a series of available methods for the investigation of in vitro microbial biofilms developed on inert substrata, as well as coated with ferrite nanoparticles, using as experimental model a Sacharomyces cerevisiae strain. Microbial biofilm architecture was directly examined by two microscopy techniques (inverted microscopy and confocal laser microscopy scanning). The in vitro study of the influence of suspended ferrite nanoparticles on planktonic cells growth, adherence and consecutive biofilm development on inert substrata was performed by using a simple microtiter method.