Mariana Viorica Bubulica, Ion Anghel, Alexandru Mihai Grumezescu*, Crina Saviuc, Georgiana Alina Anghel, Mariana Carmen Chifiriuc, Irina Gheorghe, Veronica Lazar, Alexandru Popescu: In vitro evaluation of bactericidal and antibiofilm activity of Lonicera tatarica and Viburnum opulus plant extracts on Staphylococcus strains. Revista Farmacia, 60(1):80-91, 2012.
The purpose of the present work was to evaluate the bactericidal/antibiofilm efficiency of Lonicera tatarica (LT) and Viburnum opulus (VO) extracts. The LT and VO extracts were screened for their in vitro antibacterial activity against Staphylococcus aureus and S. epidermidis reference and clinical, multidrug resistant and biofilm producing strains, isolated from ear and wound infections. The quantitative assay of the antimicrobial activity was performed by broth microdilution method in order to establish the minimal inhibitory concentration (MIC). Subsequently, the biofilm forming ability was quantified by the microtiter assay. Our results showed that the LT and VO extracts interact differently with the growth and adherence ability of S. aureus and S. epidermidis strains. LT predominantly interfered with the bacterial growth exhibiting an inhibitory effect of similar intensity against all tested strains, while VO attenuated the ability of all tested strains to colonize the inert substratum and to form biofilms. These results are leading us to the hypothesis that Lonicera tatarica and Viburnum opulus species could be used for the development of novel antimicrobial products or strategies for fighting medical biofilms.