Valentina Grumezescu, Gabriel Socol, Alexandru Mihai Grumezescu*, Alina Maria Holban, Anton Ficai, Roxana Trușcǎ, Coralia Bleotu, Paul Catalin Balaure, Rodica Cristescu, Mariana Carmen Chifiriuc: Functionalized antibiofilm thin coatings based on PLA-PVA microspheres loaded with usnic acid natural compounds fabricated by MAPLE. Applied Surface Science, 302, 262-267, 2014.
We report the fabrication of thin coatings of PLA–PVA microspheres loaded with usnic acid by matrixassisted pulsed laser evaporation (MAPLE) onto Ti substrate. The obtained coatings have been physico-chemically characterized by scanning electron microscopy (SEM) and infrared microscopy (IRM). In vitrobiological assays have been performed in order to evaluate the influence of fabricated microsphere thincoatings on the Staphylococcus aureus biofilm development as well as their biocompatibility. SEM micro-graphs have revealed a uniform morphology of thin coatings, while IRM investigations have proved boththe homogeneity and functional groups integrity of prepared thin coatings. The obtained microsphere-based thin coatings have proved to be efficient vehicles for usnic acid natural compound with antibiofilmactivity, as demonstrated by the inhibitory activity on S. aureus mature biofilm development, openingnew perspectives for the prevention and therapy associated to biofilm related infections.