Cristina Croitoru, Mariana Carmen Chifiriuc, Alexandru Mihai Grumezescu, Veronica Lazar: Fabrication and characterization of bacterial exopolysaccharides microcapsules for antibiotic drug delivery. Biointerface Research in Applied Chemistry, 3, 643-652, 2013.
A great number of bacterial extracellular polysaccharides (EPS) have been isolated and characterized in the last decades, but only a few have reached the industrial development stage for food, pharmaceutical and other industries applications. The purpose of this study was to characterize and formulate at microscale level the bacterial EPS extracted from the Gram-negative Klebsiella pneumoniae and Pseudomonas aeruginosa strains cultures, in order to used them as carriers for different antibiotics. Prepared EPS was characterized by XRD, FT-IR and SEM. The low cytoxicity on HeLa cell line, as well as the results of the qualitative and quantitative antimicrobial activity assay are proving the potential of the polysaccharidic extracts for the encapsulation and for the slow and prolonged release of antibiotics in active form. The results demonstrate the effectiveness of bacterial EPS polymers for the development of new carriers of antibiotics. However, further studies are required to optimize the obtained systems by characterizing the specific interactions between the EPS matrix and the active substances, as well as by establishing their in vitro and in vivo bioavailability.